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A new approach to the fluorometric sensing of phosphine compounds is described. The method exploits the intrinsic fluorescent reactivity of the phosphine oxide analogues obtained upon the cleavage of the P-O bond in triphenylphosphine and its derivatives. The resulting phosphine oxides show a strong enhancement in fluorescence upon reaction with methylene blue as a metal-free fluorescent quencher. Fluorescence quenching requires the interaction of phosphine oxides to the metal-free fluorescent quencher, which is characterized by an association constant of up to 4(4) M(-1). The quenching efficiency is dependent on the oxidation state of the quencher, which is shown by a change in the spectral properties of the quencher and the fluorescence of the phosphine oxide. The quenching mechanism is confirmed by computing the vibrational spectra of the metal-free fluorescent quencher-phosphine oxide adduct. The developed method is applied to the detection of triphenylphosphine and its derivative in the femtomolar range and is extended to the detection of phosphine derivatives in biological fluids. on the inside, which correlates with the date of issue. Examples of this method include a ring of gold on the inside of an older case, as an inscription, an inlaid gem or a mother-of-pearl inlay, as well as an individual reference mark on an inside of a case for a particular item. It is possible to determine the date of issue of items that are not in date rings, if the item -starterset-crack-free-download-2022/


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